目的 研究益气养阴方(SQNY)对肺癌小鼠经顺铂处理后肠道微生态的影响及可能的调控机制。方法 建立C57BL/6小鼠Lewis肺癌模型;分别给予生理盐水(NC组)、顺铂(DDP组)、CAP方案(CAP组)、DDP+SQNY;观察各组动物肿瘤体积、体重以及肠道粪便菌群比例、血清白细胞介素(IL)-6、IL-10、肿瘤坏死因子-α(TNF-α)含量;采用流式细胞术法检测各组动物脾脏组织中树突状细胞(DC)和巨噬细胞的含量。结果 自给药3 d后,DDP组、CAP组、DDP+SQNY组小鼠肿瘤体积明显小于NC组,且DDP+SQNY组小鼠肿瘤体积和最终瘤重最小(P<0.05)。NC组和DDP+SQNY组动物体重变化率基本一致,均高于DDP组和CAP组小鼠(P<0.05)。经ELISA检测,DDP+SQNY组小鼠血清IL-6、IL-10及TNF-α含量明显低于NC组、DDP组和CAP组小鼠(P<0.05)。DDP+SQNY组小鼠粪便菌群丰度明显低于其他3组,且在门水平,厚壁菌门比例较其他3组明显升高,拟杆菌门比例明显减低;在属水平,乳酸杆菌比例明显升高(P<0.05)。DDP+SQNY组小鼠脾脏内DC数量以及巨噬细胞M1/M2比值明显高于其他3组(P<0.05)。结论 SQNY可提高DDP治疗后肺癌小鼠抗肿瘤免疫功能,改变肠道菌群的组成,从而降低机体炎症水平,配合化疗药物减毒增效。
Abstract
OBJECTIVE To discuss the effect and mechanism of supplementing qi and nourishing yin(SQNY) decoction on intestinal microbiome of mice with Lewis lung carcinoma after treated with cisplatin. METHODS The model of C57BL/6 mice with lung carcinoma xenograft was established by subcutaneous seeding of Lewis cells and randomly divided into 4 groups (n=16), including saline group (NC group), cisplatin group (DDP group), CAP group (CAP group) and SQNY decoction combined with cisplatin group (DDP+SQNY group). The tumor sizes, weights and intestinal microbiota were detected. The serum levels of IL-6, IL-10, and TNF-α were detected by ELISA. The dendritic cell (DC) and macrophage subtype in spleen of mice models were detected by flow cytometry. RESULTS ①The mean tumor sizes of mice in DDP group, CAP group, DDP+SQNY groups were all smaller than NC group (P<0.05). And the mean tumor size and weight in DDP+SQNY group were less than the other three groups (P<0.05). The mean animal weights in NC or DDP+SQNY group were higher than those in DDP group and CAP group (P<0.05). ②The mean levels of serum IL-6, IL-10 and TNF-α in DDP+SQNY group were less than those in the other three groups (P<0.05). ③The mean microbiota richness in DDP+SQNY group were less than those in the other three groups (P<0.05). The proportion of fecal microbiota was significantly changed in DDP+SQNY group, including upregulation of Firmicutes phylum and Bifidobacterium Genus, and downregulation of Bacteroidetes phylum(P<0.05). ④The amount of DC and M1/M2 macrophage subtype in spleen of DDP+SQNY group mice models were more than those in the other three groups (P<0.05). CONCLUSION It′s suggested that SQNY decoction effectively enhances the anticancer effect of cisplatin by increasing the immune function and changing the intestinal microbiota.
关键词
益气养阴方 /
肺癌 /
肠道微生态 /
炎症 /
免疫功能
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Key words
supplementing qi and nourishing yin decoction /
lung carcinoma /
intestinal microflora /
inflammation /
immune function
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中图分类号：
R965
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参考文献
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脚注
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基金
浙江省中医药科技计划项目资助(2015ZA163);浙江省自然科学基金/青年基金项目资助(LQ18H270002)
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